Basic Fibroblast Growth Factor-chitosan Carriers Induce Bone Marrow-Derived Mesenchymal Stem Cells to Differentiate into Nerve Cells / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 329-333, 2011.
Article
en Zh
| WPRIM
| ID: wpr-953824
Biblioteca responsable:
WPRO
ABSTRACT
@#ObjectiveTo explore the effect of bFGF-chitosan carriers on inducing bone marrow-derived mesenchymal stem cells (MSCs) to differentiate into nerve cells.MethodsMSCs were detected by immunohistochemistry and Western blot after they were induced by bFGF-chitosan carriers to differentiate into neurons. The MTT chromometry assay was carried out to determine cell viability.ResultsThe proportion of express neural stem cells marker Nestin, and neuronal markers class Ⅲ β-tubulin and MAP-2 was 83.54% after MSCs induced by bFGF-chitosan carriers.ConclusionbFGF-chitosan carriers can induce MSCs to differentiate into nerve cells with a high percentage.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Año:
2011
Tipo del documento:
Article