Deep chemical identification of phytoecdysteroids in Achyranthes bidentata Blume by UHPLC coupled with linear ion trap-Orbitrap mass spectrometry and targeted isolation / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 551-560, 2022.
Article
en En
| WPRIM
| ID: wpr-939920
Biblioteca responsable:
WPRO
ABSTRACT
Achyranthes bidentata Blume is widely used as a traditional Chinese medicine with the effects of nourishing the liver and kidneys and strengthening muscles and bones. In this work, a rapid and simple strategy was developed for characterizing phytoecdysteroids by ultra-high-performance liquid chromatography coupled with liner ion trap-Orbitrap mass spectrometry using electrospray ionization in the negative mode. As a result, 47 phytoecdysteroids were unambiguously or tentatively characterized. Among them, seven known compounds were identified according to the reference standards along with molecular formula, retention time and fragmentation patterns, while others were mostly potential new compounds. Through targeted isolation, the structures of three new compounds were determined by NMR spectra, which were consistent with LC-MS characterization. The present study provides an efficient method to deeply characterize phytoecdysteroids.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Espectrometría de Masas
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masa por Ionización de Electrospray
/
Achyranthes
/
Cromatografía de Gases y Espectrometría de Masas
/
Medicina Tradicional China
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2022
Tipo del documento:
Article