Comparison of the prevalence of 4 periodontopathogens in supra- and subgingival plaque of young adults without periodontitis / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
; : 159-166, 2003.
Article
en Ko
| WPRIM
| ID: wpr-93680
Biblioteca responsable:
WPRO
ABSTRACT
The purpose of this study was to investigate and compare the frequence of 4 periodontal pathogens in the supra- and subgingival plaque in periodontally healthy subjects. Twenty adult individuals aged 22 to 28 years (mean age 23.65 years) participated in this study. All subjects had no pocket sites more than 3 mm deep, and the sites selected for sampling were all negative for bleeding. After drying and isolation of the sites with cotton rolls, supragingival plaque was sampled using sterile periodontal curette. Each plaque sample was placed in individual tubes containing 500 ml of 1X PBS. After removal of the supragingival sample and any remaining supragingival plaque, subgingival plaque samples were taken from the same sites using sterile curette and placed in similar individual tubes. Identification of 4 putative periodontal pathogens from the samples was performed by polymerase chain reaction based on 16S rDNA. Chi-square test was employed to identify significant explanatory variables for the presence of the 4 periodontal pathogens. The data show that Actinobacillus actinmycetemcomitans, Porphyromonas gingivalis, Bacteroides forsythus, and Fusobacterium nucleatum occurred in 16.9%, 14.4%, 52.5%, and 80.6%, respectively. No significant differences were noted in the periodontal pathogens between supra- and subgingival plaques according to the kind of teeth. However, the incisors were at higher risk for harboring F. nucleatum (p <0.05). Conclusion: These results reveal that anaerobic periodontal pathogens can be detected in supragingival plaques. Supragingival plaque may function as a reservoir of periodotopathogens.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Periodontitis
/
Diente
/
Bacteroides
/
ADN Ribosómico
/
Actinobacillus
/
Reacción en Cadena de la Polimerasa
/
Prevalencia
/
Fusobacterium nucleatum
/
Aggregatibacter actinomycetemcomitans
/
Porphyromonas gingivalis
Tipo de estudio:
Prevalence_studies
Límite:
Adult
/
Humans
Idioma:
Ko
Revista:
The Journal of the Korean Academy of Periodontology
Año:
2003
Tipo del documento:
Article