Direct protection of cultured neurons from ischemia-like injury by minocycline / 대한해부학회지
Anatomy & Cell Biology
; : 325-331, 2010.
Article
en En
| WPRIM
| ID: wpr-93237
Biblioteca responsable:
WPRO
ABSTRACT
Minocycline, a tetracycline antibiotic, is now known to protect cells via an anti-inflammatory mechanism. We further explored this effect using an in vitro model of ischemia-like injury to neurons. Coculturing neurons with microglia, the brain's resident immune cell, modestly increased cell death due to oxygen and glucose deprivation (OGD), compared to neurons alone. Treatment of cocultures with minocycline decreased cell death to a level significantly lower than that of neurons alone. Treatment of cocultures with minocycline or inhibitors of various immune mediators, also led to decreased cell death. Importantly, treatment of neuron cultures without added microglia with these same inhibitors of tissue plasminogen activator, matrix metalloproteinases, TNF-alpha and inducible nitric oxide synthase as well as minocycline also led to decreased cell death. Thus, anti-inflammatory treatments appear to be directly protective of neurons from in vitro ischemia.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Oxígeno
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Tetraciclina
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Factor de Necrosis Tumoral alfa
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Muerte Celular
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Activador de Tejido Plasminógeno
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Microglía
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Técnicas de Cocultivo
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Metaloproteinasas de la Matriz
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Óxido Nítrico Sintasa de Tipo II
/
Glucosa
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Anatomy & Cell Biology
Año:
2010
Tipo del documento:
Article