Quantitative Proteomics Using Isobaric Labeling: A Practical Guide / 基因组蛋白质组与生物信息学报·英文版
Genomics, Proteomics & Bioinformatics
; (4): 689-706, 2021.
Article
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| WPRIM
| ID: wpr-928920
Biblioteca responsable:
WPRO
ABSTRACT
In the past decade, relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples. Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions, its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results. Here, we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method. We also discuss which precautions should be taken at each step of the isobaric labeling workflow, to obtain reliable quantification results in large-scale quantitative proteomics experiments. In the last section, we discuss the broad applications of the isobaric labeling technology in biological and clinical studies, with an emphasis on thermal proteome profiling and proteogenomics.
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Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Reproducibilidad de los Resultados
/
Proteoma
/
Proteómica
/
Espectrometría de Masas en Tándem
Idioma:
En
Revista:
Genomics, Proteomics & Bioinformatics
Año:
2021
Tipo del documento:
Article