Construction of protoplast genetic transformation system for Mycena--symbiont of Gastrodia elata / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 2304-2308, 2022.
Article
en Zh
| WPRIM
| ID: wpr-928108
Biblioteca responsable:
WPRO
ABSTRACT
Mycena, a symbiont of Gastrodia elata, promotes seed germination of G. elata and plays a crucial role in the sexual reproduction of G. elata. However, the lack of genetic transformation system of Mycena blocks the research on the interaction mechanism of the two. In order to establish the protoplast transformation system of Mycena, this study analyzed the protoplast enzymatic hydrolysis system, screened the resistance markers and regeneration medium, and explored the transient transformation. After hydrolysis of Mycena hyphae with complexes enzymes for 8 h and centrifugation at 4 000 r·min~(-1), high-concentration and quality protoplast was obtained. The optimum regeneration medium for Mycena was RMV, and the optimum resistance marker was 50 mg·mL~(-1) hygromycin. The pLH-HygB-HuSHXG-GFP-HdSHXG was transformed into the protoplast of Mycena which then expressed GFP. The established protoplast transformation system of Mycena laid a foundation for analyzing the functional genes of Mycena and the molecular mechanism of the symbiosis of Mycena and G. elata.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Protoplastos
/
Simbiosis
/
Transformación Genética
/
Agaricales
/
Gastrodia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2022
Tipo del documento:
Article