The E248R protein of African swine fever virus inhibits the cGAS-STING-mediated innate immunity / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1837-1846, 2022.
Article
en Zh
| WPRIM
| ID: wpr-927821
Biblioteca responsable:
WPRO
ABSTRACT
We researched the mechanism of African swine fever virus (ASFV) protein E248R in regulating the cGAS-STING pathway. First, we verified via the dual-luciferase reporter assay system that E248R protein inhibited the secretion of IFN-β induced by cGAS-STING or HT-DNA in a dose-dependent manner. The relative quantitative PCR analysis indicated that the overexpression of E248R inhibited HT-DNA-induced transcription of IFN-b1, RANTES, IL-6, and TNF-α in PK-15 cells. Next, we found that E248R interacted with STING by co-immunoprecipitation assay and laser confocal microscopy. Finally, we demonstrated that E248R inhibited the expression of STING protein by using Western blotting. We demonstrated for the first time that the E248R protein of ASFV suppressed the host innate immune response via inhibiting STING expression. The results are pivotal in extending the understanding of the ASFV immune escape and can guide the design of vaccines against ASFV.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Porcinos
/
ADN
/
Transducción de Señal
/
Virus de la Fiebre Porcina Africana
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Inmunidad Innata
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Nucleotidiltransferasas
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2022
Tipo del documento:
Article