Development of a Unique Mouse Intervertebral Disc Degeneration Model Using a Simple Novel Tool
Asian Spine Journal
; : 415-423, 2021.
Article
en En
| WPRIM
| ID: wpr-889558
Biblioteca responsable:
WPRO
ABSTRACT
Methods@#A total of 18 mice were divided into injured (n=12) and non-injured (n=6) groups. The disc height index (DHI%) at coccygeal 4–5 level was measured by computed tomography (CT) scan for all mice. Coccygeal 4–5 discs of the injury group were injured using a 32G needle fixed to a novel tool and confirmed by CT. The non-injury group underwent no procedure. DHI% was measured by CT at 2-, 4-, and 6-week post-injury, and all mice tails were sectioned for histopathology grading of disc degeneration at the respective time intervals. @*Results@#The injured group showed significant variation in DHI% at 2, 4, and 6 weeks, whereas there was no change in the noninjured group. Histopathologic evaluation with Safranin O stain showed a worsening of the disc degeneration score at 2, 4, and 6 weeks in the injured group, but in the non-injured group there was no change. Percutaneous needle injury technique with our novel tool provided 100% accuracy and uniform degeneration. @*Conclusions@#A simple, easily reproducible mouse model for disc degeneration was created using a simple, cost-effective, novel tool and technique, its advantage being high precision and user friendly.
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Base de datos:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Asian Spine Journal
Año:
2021
Tipo del documento:
Article