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Copy number alteration profiling facilitates differential diagnosis between ossifying fibroma and fibrous dysplasia of the jaws / 国际口腔科学杂志·英文版
Article en En | WPRIM | ID: wpr-888698
Biblioteca responsable: WPRO
ABSTRACT
Ossifying fibroma (OF) and fibrous dysplasia (FD) are two fibro-osseous lesions with overlapping clinicopathological features, making diagnosis challenging. In this study, we applied a whole-genome shallow sequencing approach to facilitate differential diagnosis via precise profiling of copy number alterations (CNAs) using minute amounts of DNA extracted from morphologically correlated microdissected tissue samples. Freshly frozen tissue specimens from OF (n = 29) and FD (n = 28) patients were obtained for analysis. Lesion fibrous tissues and surrounding normal tissues were obtained by laser capture microdissection (LCM), with ~30-50 cells (5 000-10 000 µm
Asunto(s)
Texto completo: 1 Base de datos: WPRIM Asunto principal: Proteínas Nucleares / Fibroma Osificante / Diagnóstico Diferencial / Variaciones en el Número de Copia de ADN / Displasia Fibrosa Ósea / Galactosiltransferasas / Maxilares / Recurrencia Local de Neoplasia Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: International Journal of Oral Science Año: 2021 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Asunto principal: Proteínas Nucleares / Fibroma Osificante / Diagnóstico Diferencial / Variaciones en el Número de Copia de ADN / Displasia Fibrosa Ósea / Galactosiltransferasas / Maxilares / Recurrencia Local de Neoplasia Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: International Journal of Oral Science Año: 2021 Tipo del documento: Article