Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 111-121, 2021.
Article
en Zh
| WPRIM
| ID: wpr-879256
Biblioteca responsable:
WPRO
ABSTRACT
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Porcinos
/
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
/
Sistemas CRISPR-Cas
/
Edición Génica
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2021
Tipo del documento:
Article