Whole genome sequencing of SARS-CoV-2 isolated in Guangdong Province and factors influencing the sequencing / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 174-177, 2020.
Article
en Zh
| WPRIM
| ID: wpr-871264
Biblioteca responsable:
WPRO
ABSTRACT
Objective:To obtain the genome sequences of SARS-CoV-2 in respiratory specimens in Guangdong Province with next-generation sequencing (NGS) and analyze the factors influencing sequencing.Methods:Eight upper and lower respiratory tract specimens were collected from patients with SARS-CoV-2 infection in Guangdong Province in January 2020. RNA library construction was used to obtain the genome sequences of SARS-CoV-2. A bio-informatics software package (CLC Genomics Workbench 12.0) was used to analyze and compare the genomic sequences.Results:Five SARS-CoV-2 genome sequences were obtained from the eight specimens and two were obtained from lower respiratory tract specimens. The nucleotide homology to SARS-CoV-2 was 97.74%-99.90%. The Ct values were lower, while the sequencing depth, coverage, relative abundance and genome integrity were higher in sequencing the SARS-CoV-2 in lower respiratory tract specimens.Conclusions:The low Ct value of SARS-CoV-2 in the samples was good for sequencing.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Microbiology and Immunology
Año:
2020
Tipo del documento:
Article