1H-NMR-based metabolomics study on antiproliferative effect of matrine in human hepatoma cells / 中草药
Chinese Traditional and Herbal Drugs
; (24): 4275-4283, 2017.
Article
en Zh
| WPRIM
| ID: wpr-852464
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the proliferation inhibitory effect of matrine in SMMC-7721 cells using 1H-NMR metabolomics approach. Methods CCK8 method was used to detect the proliferation inhibitory effect of matrine in SMMC-7721 cells, and the cell lysates and cell culture supernatants were detected by 1H-NMR combined with multivariate statistical analysis and metabolic pathway analysis with the aim of exploring its mechanism of action. Results Matrine at the concentration of 1, 2, and 4 mg/mL could significantly inhibit the proliferation of SMMC-7721 cells. Compared with control group, 21 differential metabolites were confirmed inside and outside the cells after treatment of matrine. Matrine could significantly regulate the contents of differential metabolites such as leucine, valine and glycine inside and outside the cells, and regulate the amino acid metabolism of hepatoma cells. Conclusion Matrine plays anti-hepatoma effect likely through regulation of amino acid metabolism and energy metabolism. This study provides a scientific basis for elucidation of the anti-hepatoma mechanism of matrine from the perspective of metabolomics.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Traditional and Herbal Drugs
Año:
2017
Tipo del documento:
Article