Objective: To analyze the expression of long non-coding RNA (lncRNA) nuclear receptor subfamily 2 group F member 2 (NR2F2)-antisense RNA 1 (AS1) in human epithelial ovarian cancer. Methods: Agilent human lncRNA microarray was used to detect the expressions of lncRNA and mRNA in 3 pairs of ovarian cancer tissues and para-cancerous tissues. The abnormally expressions of mRNAs and lncRNAs were screened. Real-time fuorescent quantitative PCR was performed to detect the expression levels of lncRNA NR2F2-AS1, small nucleolar RNA host gene 4(SNHG4), LOC101927905 and OVE5-21006 in 22 ovarian cancer tissues and 10 benign ovarian cyst tissues. Moreover, the expression of NR2F2 (an antisense gene of NR2F2-AS1) in ovarian cancer SKOV3 cells after transfection with the specific siRNA targeting NR2F2-AS1 was detected by real-time fuorescent quantitative PCR. Results: Based on microarray analysis, a total of 33 403 lncRNAs and 20 351 mRNAs with different expression levels were screened from 3 pairs of epithelial ovarian cancer and paracancerous tissues. The expression level of NR2F2-AS1 in ovarian cancer tissues was lower than that in benign ovarian cyst tissues (P = 0.003 5). The expression level of NR2F2-AS1 in patients with ovarian cancer of stage I-II was lower than that of stage III-IV (P = 0.042 2). After the transfection with NR2F2-AS1 siRNA, the expression level of NR2F2 mRNA was downregulated in SKOV3 cells (P = 0.049 5). Conclusion: The expression profiles of lncRNAs in ovarian cancer tissues are significantly different from those in the corresponding para-cancerous tissues. NR2F2-AS1 is downregulated in ovarian cancer tissues, and is related to the pathological stage of ovarian cancer. After inhibiting the expression of NR2F2-AS1 in ovarian cancer SKOV3 cells, the expression level of its antisense gene NR2F2 is decreased.