Comparson of the immunogenicity of genotypeⅠJapanese encephalitis virus subunit vaccine candidate antigens / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1314-1322, 2020.
Article
en Zh
| WPRIM
| ID: wpr-826845
Biblioteca responsable:
WPRO
ABSTRACT
To screen the best genotypeⅠJapanese encephalitis virus subunit vaccine candidate antigens, the prMEIII gene, the polytope gene and the prMEIII-polytope fusion gene of the GenotypeⅠJapanese encephalitis virus GS strain were cloned into prokaryotic expression vector pET-30a. The recombinant proteins were obtained after the induction and purification. The prepared recombinant proteins were immunized to mice, and the immunogenicity of the subunit vaccine candidate antigens was evaluated through monitoring the humoral immune response by ELISA, detecting the neutralizing antibody titer by plaque reduction neutralization test, and testing the cell-mediated immune response by lymphocyte proliferation assay and cytokine profiling. The recombinant proteins with the molecular weights of 35 (prMEIII), 28 (polytope antigen) and 57 kDa (prMEIII-polytope) induced strong humoral and cellular immune responses in mice. Compared with prMEIII-polytope and polytope proteins, the prMEIII protein induced a significant expression of IL-2 and IFN-γ (P0.05). The study suggests that the prMEIII protein can be used for the development of the Japanese encephalitis virus subunit vaccine.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Sangre
/
Vacunas Virales
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Encefalitis Japonesa
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Vacunas de Subunidad
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Virus de la Encefalitis Japonesa (Especie)
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Alergia e Inmunología
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Inmunogenicidad Vacunal
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Ratones Endogámicos BALB C
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Anticuerpos Antivirales
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Antígenos Virales
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2020
Tipo del documento:
Article