Effect of extracellular ubiquitin on the proliferation, invasion, and migration of hepatoma cells / 临床肝胆病杂志
Journal of Clinical Hepatology
; (12): 1289-1292, 2020.
Article
en Zh
| WPRIM
| ID: wpr-822188
Biblioteca responsable:
WPRO
ABSTRACT
ObjectiveTo investigate the effect of extracellular ubiquitin on the proliferation, invasion, and migration of hepatoma cells by in vitro cell experiments. MethodsThe hepatoma cells (HepG2) were treated with extracellular ubiquitin at different concentrations (200, 400, and 800 ng/ml); CCK-8 assay was used to observe cell proliferation, Transwell assay was used to observe the effect of extracellular ubiquitin at different concentrations on the invasion ability of hepatoma cells, and wound healing assay and Western blotting were used to observe the effect of extracellular ubiquitin on the migration ability of hepatoma cells. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsThe CCK-8 assay showed that extracellular ubiquitin significantly promoted the proliferation of hepatoma cells in a concentration-dependent manner, with the most significant effect at the concentration of 400 ng/ml, and the intervention group had a significantly higher relative absorbance value than the control group at 48, 72, and 96 hours (all P＜0.05). The Transwell assay showed that different concentrations of extracellular ubiquitin significantly promoted the invasion of hepatoma cells, with the most significant effect in the group treated by 400 ng/ml extracellular ubiquitin (134.00±8.18 vs 347.33±18.90, P＜0.001). The Wound healing assay and Western blotting showed that 400 ng/ml extracellular ubiquitin significantly increased the migration ability of HepG2 cells. ConclusionExtracellular ubiquitin can significantly promote the proliferation, invasion, and migration of HepG2 cells in vitro in a concentration-dependent manner.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Clinical Hepatology
Año:
2020
Tipo del documento:
Article