Cellular toxicity of isoniazid together with rifampicin and the metabolites of isoniazid on QSG-7701 hepatocytes
Asian Pacific Journal of Tropical Medicine
; (12): 306-309, 2012.
Article
en En
| WPRIM
| ID: wpr-819781
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the cellular toxicity of isoniazid together with rifampicin and the metabolites of isoniazid on cultured QSG-7701 cells lines.@*METHODS@#Isoniazid, rifampicin, mixture of rifampicin and isoniazid, acetylhydrazine, hydrazine were added in cultural media of QSG-7701 cells and cultured for 48 hours. The survival rate of cells was determined by MTT method. The cultural media and cells were collected and the activity of lactate dehydrogenase was detected by chromatometry.@*RESULTS@#Compared with control group, the survival rate decreased significantly and the lactate dehydrogenase released from cell increased significantly in cells treated with isoniazid, rifampicin, acetylhydrazine, hydrazine. Hydrazine, the metabolite of isoniazid produced significant damage on hepatocytes in low concentration.@*CONCLUSIONS@#Rifampicin together with rifampicin and metabolites of isoniazid produce cellular toxic effects and hydrazine may be the most toxiferous metabolite.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Rifampin
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Estudios de Casos y Controles
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Supervivencia Celular
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Células Cultivadas
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Análisis de Varianza
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Hepatocitos
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Combinación de Medicamentos
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Toxicidad
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Enfermedad Hepática Inducida por Sustancias y Drogas
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Isoniazida
Tipo de estudio:
Observational_studies
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Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2012
Tipo del documento:
Article