Simultaneous Determination of the Contents of 6 Volatile Components in Zhachong Shisan by GC / 中国药房
China Pharmacy
; (12): 1650-1654, 2019.
Article
en Zh
| WPRIM
| ID: wpr-817115
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE: To establish a method for simultaneously determining the contents of 6 volatile components such as eugenol, methyl eugenol, isoeugenol, α-asarone, alkaloid and myristyl ether in Zhachong shisan. METHODS:Volatile oil was extracted by steam distillation and analyzed by gas chromatography. The chromatographic column was ov-1701, with hydrogen flame ionization detector. The carrier gas was nitrogen, the inlet temperature was 220 ℃, the detector temperature was 240 ℃, the flow rate was 1.0 mL/min, the injection volume was 1 μL, the split ratio was 10 ∶ 1, with programmed temperature. RESULTS: The linear ranges of eugenol, methyl eugenol, isoeugenol, α-asarone, costunolide and myristyl were 2.00-12.00, 1.00-6.00, 0.60-3.60, 0.05-0.30, 0.05-0.30, 0.60-3.60 mg/mL(r=0.999 9). The detection limits were 0.01, 0.01, 0.02, 0.01, 0.01, 0.01 mg/mL, respectively; the limits of quantification were 0.05, 0.03, 0.06, 0.02, 0.02, 0.03 mg/mL, respectively. RSDs of precision, stability and reproducible tests were all lower than 3%; average recovery rates were 99.33%-100.04%, RSDs were 0.02%-0.35% (n=6); RSDs of durability test were all lower than 5%. CONCLUSIONS: The established method was simple and reproducible. It can be used for simultaneous determination of the contents of 6 volatile components in Zhachong shisan.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Año:
2019
Tipo del documento:
Article