Ultrasound molecular detection of immediately blood-mediated inflammatory reaction induced by
islets transplantation in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 632-638, 2015.
Article
en Zh
| WPRIM
| ID: wpr-815295
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To study the feasibility of ultrasonic molecular imaging of immediately blood-mediated inflammatory reaction (IBMIR) in vitro.
@*METHODS@#IBMIR models in vitro were divided into 3 groups: Group A, no microbubbles were added; Group B, non-targeted micro-bubbles were added; Group C, Lys-Gly-Asp-Ser (KGDS)-targeted microbubbles (MBK) were added. The ultrasonic enhancement of IBMIR in loops by ultrasonic contrast imaging was evaluated.
@*RESULTS@#The contrast-enhanced US imaging did not show thrombus formation in the group A, whereas the thrombus was found in the Group B and C with a change in filling defects or ring enhancement, respectively. The time for detecting thrombosis was (7.3 ± 0.5) min and (13.2 ± 0.6) min in Group B and Group C, respectively (P<0.05). The average-gray scales of thrombus in Group B and Group C were 31.22 ± 3.56 and 75.85 ± 5.21, respectively (P<0.05). The fluorescence microscope also showed that MBK was attached to thrombus surrounding islets.
@*CONCLUSION@#IBMIR model in vitro showed that KGDS-targeted ultrasound contrast agent could adhere to thrombus shell surrounding islets and molecular target ultrasonography could image these thrombi noninvasively and effectively.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Trombosis
/
Diagnóstico por Imagen
/
Trasplante de Islotes Pancreáticos
/
Ultrasonografía
/
Medios de Contraste
/
Microburbujas
/
Inflamación
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2015
Tipo del documento:
Article