Influence of BRAF interference on SW579 cell lines in thyroid cancer / 中南大学学报(医学版)
Zhongnan Daxue xuebao. Yixue ban
; (12): 576-581, 2013.
Article
en Zh
| WPRIM
| ID: wpr-814840
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To determine the influence of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) interference on SW579 cell lines in thyroid cancer.@*METHODS@#We designed 2 pairs of siRNA interference sequences, transfected them into SW579 cell line with liposome, and detected the interference with RT-PCR method. For successfully interfered cell lines, the changes in cell proliferation, cell cycle, and the expression of related proteins in mitogen-activated protein signal-regulated kinase /extracellular signal-regulated kinase (MEK/ ERK) signal pathway were detected.@*RESULTS@#After 2 pairs of siRNA transfection, the expressions of BRAF mRNA and protein of SW579 cell lines were significantly inhibited (P<0.01). The proliferation was inhibited, the cell cycle was changed, G1/S phase increased, and MEK/ERK signal pathway was inhibited.@*CONCLUSION@#Inhibition of growth and proliferation of SW579 cell lines by BRAF may be functioned by de-activating MEK/ERK signal pathway.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Neoplasias de la Tiroides
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Sistema de Señalización de MAP Quinasas
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ARN Interferente Pequeño
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Interferencia de ARN
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Línea Celular Tumoral
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Proteínas Proto-Oncogénicas B-raf
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Proliferación Celular
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Genética
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Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Zhongnan Daxue xuebao. Yixue ban
Año:
2013
Tipo del documento:
Article