Identification of a highly conserved domain that suppresses the DNA-binding domain-DNA interactions in the androgen receptor / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 394-398, 2005.
Article
en Zh
| WPRIM
| ID: wpr-813552
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To determine the gene regulation of androgen receptor (AR).@*METHODS@#Gel shift assay, protein-protein pull down assay, western blot and technique of site-directed mutagenesis were used to study the gene regulation of AR.@*RESULTS@#The N terminal of AR contained an inhibitory domain located in an 81-amino acid segment lying upstream of the DNA-binding domain (DBD). The inhibitory domain interacted directly with DBD and repressed DBD binding to the ARE. Mutations of the conserved amino acid residues (K520E and R538E) within the inhibitory domain decreased its inhibiting ability in vitro and increased AR trans-activation in vivo.@*CONCLUSION@#These data demonstrated the existence of a novel inhibitory domain in the N-terminal part of AR, which might play important role in the regulation of AR trans-activation.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
ADN
/
Transfección
/
Receptores Androgénicos
/
Activación Transcripcional
/
Química
/
Regulación de la Expresión Génica
/
Elementos de Respuesta
/
Proteínas de Unión al ADN
/
Genética
/
Metabolismo
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Humans
/
Male
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2005
Tipo del documento:
Article