Gene transfection efficiency and destination of recombinant adenovirus gene transfer through internal jugular vein injection in rats / 中南大学学报(医学版)
Zhongnan Daxue xuebao. Yixue ban
; (12): 653-656, 2005.
Article
en Zh
| WPRIM
| ID: wpr-813431
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the adenovirus-mediated LacZ gene expression and the destination in different organs of SD rats after the intravenous injection in rats.@*METHODS@#Recombinant adenovirus vector containing LacZ was transferred to SD rats by injecting into the internal jugular vein. To identify the sites and periods of LacZ gene expression, X-gal staining was used to detect beta-gal level and period of LacZ gene expression of different organs in the transfected and non-transfected rats at different time intervals.@*RESULTS@#On the 1st day after the injection, the lung, liver, kidney, and spleen expressed some beta-gal; on the 3rd day after the injection, the lung, liver, kidney, and spleen expressed beta-gal obviously; their peak levels were on the 7th day; the beta-gal level decreased on the 14th day; beta-gal expression disappeared in the most organs except the lungs on the 28th day. In all animals, the brain did not express any beta-gal.@*CONCLUSION@#The adenovirus-mediated exogenous gene transfer in the internal jugular vein may be an effective approach of gene therapy in some diseases in the lung, liver, and kidney.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteínas Recombinantes
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Transfección
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Terapia Genética
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Distribución Aleatoria
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Adenoviridae
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Ratas Sprague-Dawley
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Técnicas de Transferencia de Gen
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Genética
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Inyecciones Intravenosas
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Venas Yugulares
Límite:
Animals
Idioma:
Zh
Revista:
Zhongnan Daxue xuebao. Yixue ban
Año:
2005
Tipo del documento:
Article