Excessive fluoride increases the expression of osteocalcin in the mouse testis / 中华男科学杂志
Zhonghua nankexue
; Zhonghua nankexue;(12): 782-785, 2017.
Article
en Zh
| WPRIM
| ID: wpr-812879
Biblioteca responsable:
WPRO
ABSTRACT
Objective@#To observe the influence of excessive fluoride on the levels of osteocalcin and testosterone in the testis of the male mouse.@*METHODS@#Twenty-four C57BL/6J male mice were equally randomized into a normal control and a fluorosis model group, the former fed on distilled water while the latter on a solution of sodium fluoride (100 mg/L) in distilled water, both for 12 weeks. Then, the level of osteocalcin in the testis tissue was measured with the immunohistochemical streptavidin-peroxidase (SP) method and those of osteocalcin and testosterone in the serum determined by ELISA.@*RESULTS@#After 12 weeks of fluoride intervention, the level of serum osteocalcin was significantly higher in the fluorosis models than in the normal controls (［68.05 ± 5.32］ vs ［47.50 ± 5.73］ pg/mL, F = 11.901, P = 0.008), while that of testosterone markedly lower in the former than the latter group (［8.07 ± 1.35］ vs ［12.94 ± 3.09］ ng/mL, F = 2.313, P = 0.006). The results of immunohistochemical SP showed the expression of osteocalcin in the cell membrane and cytoplasm of the fluorosis models, which was evidently higher than in the normal controls.@*CONCLUSIONS@#Twelve-week intake of 100 mg/L fluoride solution can decrease the level of testosterone and increase the expression of osteocalcin in the testis of the male mouse.
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Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Fluoruro de Sodio
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Testículo
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Distribución Aleatoria
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Osteocalcina
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Toxicidad
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Intoxicación por Flúor
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Fluoruros
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Metabolismo
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua nankexue
Año:
2017
Tipo del documento:
Article