Expressions of TRPV1 and TRPA1 in the dorsal root ganglion in the rat model of orchialgia / 中华男科学杂志
National Journal of Andrology
; (12): 296-301, 2017.
Article
en Zh
| WPRIM
| ID: wpr-812770
Biblioteca responsable:
WPRO
ABSTRACT
Objective@#To explore the expressions of transient receptor potential vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in the dorsal root ganglion (DRG) and their action mechanisms in the rat model of orchialgia.@*METHODS@#The models of orchialgia were established in male SD rats by injection of 2% acetic acid into the testis. Then the number of spontaneous pain responses and withdrawal latency in the model rats were recorded by behavioral tests and the expressions of TRPV1 and TRPA1 in T13－L1 DRGs determined by RT-qPCR, Western blot and immunofluorescence staining.@*RESULTS@#Compared with the normal control rats, the orchialgia models showed a significant increase in the number of spontaneous pain responses (0.13 ± 0.35 vs 22.63 ± 3.42, P<0.01) and a decrease in the withdrawal latency at 4 hours after injection (［12.75 ± 1.50］ vs ［4.85 ± 1.00］ s, P<0.05). The mRNA expressions of both TRPV1 and TRPA1 were observed in the membrane of the neurons in the DRG, the former increased by 1.77 times and the latter by 1.75 times that of the control (P<0.05).@*CONCLUSIONS@#The expressions of TRPV1 and TRPA1 were up-regulated in the DRG of the rat models of orchialgia, which may be involved in the allodynia and hyperalgesia of the rats.
Palabras clave
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Oxidorreductasas
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Enfermedades Testiculares
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Glicoproteínas de Membrana
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Regulación hacia Arriba
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Ratas Sprague-Dawley
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Ácido Acético
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Canales Catiónicos TRPV
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Canal Catiónico TRPA1
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Ganglios Espinales
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Hiperalgesia
Límite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2017
Tipo del documento:
Article