Identification and characterization of major constituents in Juglans mandshurica using ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 525-545, 2018.
Article
en En
| WPRIM
| ID: wpr-812376
Biblioteca responsable:
WPRO
ABSTRACT
The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C column (2.1 mm × 100 mm, 1.7 μm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identifled by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Triterpenos
/
Flavonoides
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Medicamentos Herbarios Chinos
/
Estructura Molecular
/
Química
/
Reproducibilidad de los Resultados
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Bases de Datos Factuales
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Cromatografía Líquida de Alta Presión
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Naftoquinonas
/
Juglans
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2018
Tipo del documento:
Article