Effects of exogenous ghrelin on muscle atrophy in elderly mice / 中华老年医学杂志
Chinese Journal of Geriatrics
; (12): 1404-1407, 2019.
Article
en Zh
| WPRIM
| ID: wpr-800391
Biblioteca responsable:
WPRO
ABSTRACT
Objective@#To investigate the effects of acylated ghrelin and des-acylated ghrelin on skeletal muscle atrophy in elderly mice.@*Methods@#Eighteen-month-old wild type(WT)mice and ghrelin-/- mice were selected to perform body composition analysis and wheel-running test under conditions of feeding versus fasting.The gene expressions of myogenic regulatory factors including muscle differentiation factor MyoD, myogenin, atrogin-1, muscle-specific RING finger protein 1(muRF-1), and insulin growth factor 1(IGF-1)in mice gastrocnemius muscle were detected by realtime polymerase chain reaction(PCR).@*Results@#The locomotor activity during the wheel-running test were significantly lower in elderly ghrelin-/- mice than in elderly WT mice(3 929±263 times/h vs.5359±601 times/h, t=4.87, P<0.05). The gene expressions of MyoD, myogenin, atrogin-1, muRF-1 and IGF-1 had no significant difference between the two groups(P>0.05). After 48 h fasting, the decrements of body weight, fat and muscle weight were more in ghrelin-/- mice than in WT mice(P<0.05). In fasting old ghrelin-/- mice, the gene expressions of MyoD and myogenin were increased(improved)(t=232.00 and 121.00, P<0.05), and the gene expressions of atrogin-1 and muRF-1 were decreased(improved)(t=30.40 and 54.00, P<0.05)after treatment with both acylated ghrelin and desacylated ghrelin.@*Conclusions@#The acylated ghrelin and desacylated ghrelin may play protective roles in age-related muscle atrophy.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Geriatrics
Año:
2019
Tipo del documento:
Article