Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis
The Korean Journal of Parasitology
; : 327-334, 2013.
Article
en En
| WPRIM
| ID: wpr-79739
Biblioteca responsable:
WPRO
ABSTRACT
Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an alpha-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Datos de Secuencia Molecular
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Secuencia de Bases
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Regulación de la Expresión Génica
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Western Blotting
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Cromatografía Liquida
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Secuencia de Aminoácidos
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Clonación Molecular
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ADN Complementario
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Ixodidae
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Espectrometría de Masas en Tándem
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
The Korean Journal of Parasitology
Año:
2013
Tipo del documento:
Article