Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 59-61, 2016.
Article
en Zh
| WPRIM
| ID: wpr-790558
Biblioteca responsable:
WPRO
ABSTRACT
Objective To establish the method for determination content of glycine in the pharmaceutical of human coag‐ulation factor Ⅷ by HPLC .Methods The analysis was carried on a Shim‐Pack CLC‐ODS column with a mobile phase of 50%acetonitrile‐0 .05 mol/L sodium acetate buffer (35∶65) at the detection wavelength of 360 nm ,using alanine and 2 ,4‐dini‐trofluorobenzene as the internal standard and derivation agent ,respectively .Results The method showed a good linearity in the range of 0 .006‐0 .030 mg/ml (r=0 .999 3) for glycine .The average recovery was 101 .4% ,and the RSD was 0 .14% (n=9) .Conclusion This method was simple ,sensitive ,accurate ,reliable ,and suitable for determination of glycine in the pharma‐ceutical of human coagulation factor Ⅷ .
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Practice
Año:
2016
Tipo del documento:
Article