Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 153-155, 2015.
Article
en Zh
| WPRIM
| ID: wpr-790434
Biblioteca responsable:
WPRO
ABSTRACT
Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its u‐niformity and stability .Methods UPLC‐ELSD was applied to obtain a chromatogram with 11 well separated peaks ,and the chromatographic condition was as follows:mobile phase was gradient elution by acetonitrile‐water ,column was ACQUITY UPLC BEH C18 (2 .1 × 100 mm ,1 .7 μm) with the column temperature of 30 ℃ and the flow rate of 0 .2 ml/min .Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM .Similar‐ities were all greater than 0 .90 ,which could meet the requirement for chromatographic fingerprint of TCM .Conclusion The stability ,repeatability and precision of the developed method were all validated ,and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine .
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1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Practice
Año:
2015
Tipo del documento:
Article