Screening of main active components against cardiomyocyte hypoxia/reoxygen-ation injury in shexiang baoxin Pill / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 209-211,219, 2014.
Article
en Zh
| WPRIM
| ID: wpr-790316
Biblioteca responsable:
WPRO
ABSTRACT
Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Journal of Pharmaceutical Practice
Año:
2014
Tipo del documento:
Article