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Molecular Mechanism and family research of a pedigree with B(A).06 subtype / 中华医学遗传学杂志
Article en Zh | WPRIM | ID: wpr-781310
Biblioteca responsable: WPRO
ABSTRACT
OBJECTIVE@#To explore the serological and genotypic characteristics of a pedigree with B(A).06 subtype.@*METHODS@#Serological methods was used to identify the ABO phenotypes. Exons 6 and 7 of the ABO gene and flanking regions were subjected to direct sequencing and TA clonal sequencing in order to determine the genotype of individuals with inconsistent results for forward and reverse serological typing.@*RESULTS@#Among 12 individuals from 4 generations, 5 were identified with a AwB phenotype, along with a c.803C>G mutation in exon 7 of the B allele, which was named as B(A).06. The B(A).06/O.01.01 phenotype may be easily missed due to its weak anti-A antibody in the serum upon initial serological test.@*CONCLUSION@#A B(A).06 subtype family was identified. The serological phenotype of individuals carrying the B(A).06 allele may be affected by the opposite DNA strand.
Asunto(s)
Texto completo: 1 Base de datos: WPRIM Asunto principal: Linaje / Fenotipo / Sistema del Grupo Sanguíneo ABO / Mutación Puntual / Alelos / Genética / Genotipo Tipo de estudio: Prognostic_studies Límite: Humans Idioma: Zh Revista: Zhonghua Yi Xue Yi Chuan Xue Za Zhi Año: 2019 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Asunto principal: Linaje / Fenotipo / Sistema del Grupo Sanguíneo ABO / Mutación Puntual / Alelos / Genética / Genotipo Tipo de estudio: Prognostic_studies Límite: Humans Idioma: Zh Revista: Zhonghua Yi Xue Yi Chuan Xue Za Zhi Año: 2019 Tipo del documento: Article