Role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats / 中国骨伤
China Journal of Orthopaedics and Traumatology
; (12): 68-71, 2019.
Article
en Zh
| WPRIM
| ID: wpr-776137
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To study role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats.@*METHODS@#Eighteen male SD rats weighted (200±20) g were randomly divided into 2 groups, namely control and model group, and 9 in each group. Knee OA model group was established by using modified Hulth method in model group. Control group was not treated. Synovial tissue and serum was extracted at 4 and 21 d after operation. Expression of CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13 were detected by real-time PCR respectively. NF-κB p65 protein was detected by Western-blot; serum concentrations of haluronic acid (HA), N-propeptide of type III procollagen(PIIINP) was detected by Elisa.@*RESULTS@#Expression of CD14, ADAMTS-4, and NF-κB p65 in model group were higher than that of control group at 4 and 21 days after operation, while expression of TLR4, IL-1β, TNF-α and MMP-13 were higher than that of control group at 21 days after operation(<0.01). Concentration of PIIINP and HA in model group were higher than that of control group at 4 days after operation, while there was no significant difference at 21 days after operation.@*CONCLUSIONS@#NF-κB pathway could mediate occurrence of KOA by early activating and triggeringg synovial increasingly secreting inflammatory secretion CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13, PIIINP and HA.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Membrana Sinovial
/
Transducción de Señal
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FN-kappa B
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Ratas Sprague-Dawley
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Osteoartritis de la Rodilla
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Receptor Toll-Like 4
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Orthopaedics and Traumatology
Año:
2019
Tipo del documento:
Article