Proteomic Analysis on Exosomes Derived from Patients’ Sera Infected with Echinococcus granulosus
The Korean Journal of Parasitology
; : 489-497, 2019.
Article
en En
| WPRIM
| ID: wpr-761777
Biblioteca responsable:
WPRO
ABSTRACT
Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcus granulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Espectrometría de Masas
/
Donantes de Tejidos
/
Tubulina (Proteína)
/
Histonas
/
Zoonosis
/
Cuerpo Humano
/
Biología Computacional
/
Echinococcus granulosus
/
Diagnóstico
/
Equinococosis
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
The Korean Journal of Parasitology
Año:
2019
Tipo del documento:
Article