The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection
Protein & Cell
; (12): 590-600, 2017.
Article
en En
| WPRIM
| ID: wpr-756983
Biblioteca responsable:
WPRO
ABSTRACT
Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Unión Proteica
/
Receptores Virales
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Termodinámica
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Virología
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Sitios de Unión
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Proteínas Recombinantes de Fusión
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Fragmentos Fab de Inmunoglobulinas
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Modelos Moleculares
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Expresión Génica
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Línea Celular
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
Protein & Cell
Año:
2017
Tipo del documento:
Article