Effect of the combination of hyperbaric oxygen and 5-fluorouracil on the proliferation and metastasis of nasopharyngeal carcinoma CNE-2Z cell line / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 386-391, 2010.
Article
en Zh
| WPRIM
| ID: wpr-747994
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect and the possible influential mechanisms of hyperbaric oxygen (HBO) and 5-fluorouracil (5-FU) on the proliferation and metastasis in human nasopharyngeal carcinoma (NPC) CNE2Z cell line.@*METHOD@#NPC CNE2Z cell line were divided into 4 groups randomly: group A: control group; group B: 5-FU group; group C: HBO group; group D: 5-FU and HBO group. The inhibition effect of proliferation in CNE2Z cells of all groups through 24 h, 48 h and 72 h disposal was detected by MTT reduction assay; Transwell chamber assay was performed to determine the effect of HBO and (or) 5-FU on the metastasis of the CNE2Z cells; The MMP-9, VEGF expression in CNE2Z cells of all groups were detected by SP immunocytochemical stain and observe the expressed image by micro.@*RESULT@#There were statistical difference on the inhibition effect of proliferation in C group and A group after 48 h and 72 h disposal (P0.05) and between B, C group and D group (P>0.05); Average optical of the MMP-9, VEGF expression were not statistically significant difference in C group and A group (P>0.05) and between C group and D group (P>0.05).@*CONCLUSION@#Simple HBO disposal after 48 h and 72 h could inhibit the proliferation potential of NPC CNE2Z cell line, but the combination of HBO and 5-FU only after 48 h disposal could all the more inhibit the proliferation potential. Simple HBO disposal couldn't decrease the MMP-9 and VEGF high-expression and inhibit the metastasis potential in human NPC CNE2Z cell line, the combination of HBO and 5-FU disposal also couldn't further decreased the MMP-9 and VEGF high-expression and inhibited the metastasis potential.
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Base de datos:
WPRIM
Asunto principal:
Patología
/
Terapéutica
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Neoplasias Nasofaríngeas
/
Apoptosis
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Metaloproteinasa 9 de la Matriz
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Línea Celular Tumoral
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Factor A de Crecimiento Endotelial Vascular
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Usos Terapéuticos
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Proliferación Celular
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Fluorouracilo
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Año:
2010
Tipo del documento:
Article