Growth inhibition effection of perlecan anti-sense cDNA on human laryngeal carcinoma xnograft in nude mice / 临床耳鼻咽喉头颈外科杂志
Lin chuang er bi yan hou ke za zhi
; (24): 1326-1329, 2014.
Article
en Zh
| WPRIM
| ID: wpr-747692
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To observe growth inhibition effect of perlecan anti-sense cDNA (pAP) on human laryngeal carcinoma xnografted in nude mice. To vertify its antitumor effect and mechanism in vivo, and it may be useful as a biomarker in carcinoma of larynx cancer.@*METHOD@#Created the model of human laryngeal carcinoma xnograft in nude mice. To observe growth of those xnografts in nude mice and draw growth curve of xnografted. The expression of perlecan mRNA and portein in xnografts were examined by RT-PCR and immunohistochemistry.@*RESULT@#Volume of xnografts in the group transfected by the plasmids of pAP were significant small as compared with other two groups made by the wild type cells and phpApr-neol cells (P < 0.05). It was showed that the expression of perlecan mRNA and protein were significantly reduced in the tumor of pAP transfected Hep-2 cells as compared with the tumors transfected by the wild type cells and phβApr-neol cells (P < 0.01).@*CONCLUSION@#These data raise the possibility that pAP many play key roles in the growth of those xnografts in nude mice.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Plásmidos
/
Terapéutica
/
ARN Mensajero
/
Transfección
/
Neoplasias Laríngeas
/
ADN sin Sentido
/
ADN Complementario
/
Proteoglicanos de Heparán Sulfato
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Lin chuang er bi yan hou ke za zhi
Año:
2014
Tipo del documento:
Article