Additive e ffects of hyperinsulinemia and ischemia on canine myocardial GLUT4 gene expression in vivo / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 115-117, 2001.
Article
en Zh
| WPRIM
| ID: wpr-735343
Biblioteca responsable:
WPRO
ABSTRACT
Objective: To investigate whether there is additi ve effects of hyperinsulinemia and ischemia on expression of canine myocardial G LUT4 gene in vivo. Methods: The expression of myocardial GLU T4 was determined by semiquantitative immunoblotting.The expression of GLUT4 mRN A was determined by semiquantitative Northern blotting. Results: Dramatic changes were seen in GLUT4 mRNA and GLUT4 expression in the ischemic hearts.After infusing insulin for 8 h,regional GLUT4 mRNA and GLUT4 levels in is chemic hearts were 2.5, 2.3-fold that of expression in normal hearts(P<0.01 ). Myocardial glucose uptake in ischemic hearts was increased by 4-fold when co mpared with normal hearts(P<0.01). Conclusion: There are not only additive effects of hyperinsulinemia and low-flow ischemia on canine myoc ardial GLUT4 mRNA and GLUT4 expression in vivo, but also increase of myocar dial glucose uptake. Enhanced GLUT4 expression may be an important protective m echanism by which myocardial cells enhance glucose uptake and metabolism during low-flow ischemia.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Academic Journal of Second Military Medical University
Año:
2001
Tipo del documento:
Article