Production and Characterization of a New alpha-Glucosidase Inhibitory Peptide from Aspergillus oryzae N159-1
Mycobiology
; : 149-154, 2013.
Article
en En
| WPRIM
| ID: wpr-729421
Biblioteca responsable:
WPRO
ABSTRACT
An alpha-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at 27degrees C for five days. The inhibitor was purified using a series of purification steps involving ultrafiltration, Sephadex G-25 gel permeation chromatography, strong cation exchange solid phase extraction, reverse-phase high performance liquid chromatography, and size exclusion chromatography. The final yield of the purification was 1.9%. Results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the purified alpha-glucosidase inhibitor was a tri-peptide, Pro-Phe-Pro, with the molecular weight of 360.1 Da. The IC50 value of the peptide against alpha-glucosidase activity was 3.1 mg/mL. Using Lineweaver-Burk plot analysis, the inhibition pattern indicated that the inhibitor acts as a mixed type inhibitor.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Hidrolisados de Proteína
/
Aspergillus
/
Aspergillus oryzae
/
Espectrometría de Masas
/
Ultrafiltración
/
Caseínas
/
Cromatografía en Gel
/
Cromatografía Liquida
/
Dextranos
/
Concentración 50 Inhibidora
Idioma:
En
Revista:
Mycobiology
Año:
2013
Tipo del documento:
Article