Akt Expression in Acute Leukemia Cells and Its Clinical Significance / 대한혈액학회지
Korean Journal of Hematology
; : 253-260, 2003.
Article
en Ko
| WPRIM
| ID: wpr-720102
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Disturbances in apoptosis through phosphoinositide 3-kinase(PI3K)/Akt pathway is thought to be crucial in cancer cell immortality. Enhanced expression and activation of Akt was investigated in several malignancies but not in acute leukemia. We investigated the expression of Akt and phospho-Akt in acute leukemia cells and clinical characteristics of expression and non-expression group. METHODS: Bone marrow cells from patients who were newly diagnosed as acute leukemia and healthy volunteer were obtained and analyzed by Western blot analysis using monoclonal antibody against Akt, phospho-Akt (Ser473), and phospho-Akt (Thr308). Clinical data were obtained retrospectively. RESULTS: The expression of Akt was demonstrated in 27 of 43 cases (63%) and phospho- Akt(Ser473) was noted in 24 of 27 (54%) Akt-positive cases, respectively. Phospho-Akt (Ser473)-expression group showed significantly higher initial WBC counts compared to negative group (P=0.003). By chromosomal analysis, patients with Akt expression did not show any good prognostic karyotype (P=0.001). CONCLUSION: This result suggests that Akt overexpression and activation is detected in acute leukemia cells and might have a role in molecular pathogenesis of acute leukemia.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Células de la Médula Ósea
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Leucemia
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Western Blotting
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Estudios Retrospectivos
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Apoptosis
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Cariotipo
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Voluntarios Sanos
Tipo de estudio:
Observational_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Hematology
Año:
2003
Tipo del documento:
Article