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The Phosphodiesterase 4 Inhibitor Roflumilast Protects against Cigarette Smoke Extract-Induced Mitophagy-Dependent Cell Death in Epithelial Cells / 결핵및호흡기질환
Article en En | WPRIM | ID: wpr-713768
Biblioteca responsable: WPRO
ABSTRACT
BACKGROUND: Recent studies show that mitophagy, the autophagy-dependent turnover of mitochondria, mediates pulmonary epithelial cell death in response to cigarette smoke extract (CSE) exposure and contributes to the development of emphysema in vivo during chronic cigarette smoke (CS) exposure, although the underlying mechanisms remain unclear. METHODS: In this study, we investigated the role of mitophagy in the regulation of CSE-exposed lung bronchial epithelial cell (Beas-2B) death. We also investigated the role of a phosphodiesterase 4 inhibitor, roflumilast, in CSE-induced mitophagy-dependent cell death. RESULTS: Our results demonstrated that CSE induces mitophagy in Beas-2B cells through mitochondrial dysfunction and increased the expression levels of the mitophagy regulator protein, PTEN-induced putative kinase-1 (PINK1), and the mitochondrial fission protein, dynamin-1-like protein (DRP1). CSE-induced epithelial cell death was significantly increased in Beas-2B cells exposed to CSE but was decreased by small interfering RNA-dependent knockdown of DRP1. Treatment with roflumilast in Beas-2B cells inhibited CSE-induced mitochondrial dysfunction and mitophagy by inhibiting the expression of phospho-DRP1 and -PINK1. Roflumilast protected against cell death and increased cell viability, as determined by the lactate dehydrogenase release test and the MTT assay, respectively, in Beas-2B cells exposed to CSE. CONCLUSION: These findings suggest that roflumilast plays a protective role in CS-induced mitophagy-dependent cell death.
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Texto completo: 1 Base de datos: WPRIM Asunto principal: Humo / Supervivencia Celular / Muerte Celular / Enfermedad Pulmonar Obstructiva Crónica / Enfisema / Células Epiteliales / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4 / Mitofagia / Dinámicas Mitocondriales / Productos de Tabaco Idioma: En Revista: Tuberculosis and Respiratory Diseases Año: 2018 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Asunto principal: Humo / Supervivencia Celular / Muerte Celular / Enfermedad Pulmonar Obstructiva Crónica / Enfisema / Células Epiteliales / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4 / Mitofagia / Dinámicas Mitocondriales / Productos de Tabaco Idioma: En Revista: Tuberculosis and Respiratory Diseases Año: 2018 Tipo del documento: Article