Mechanism of platelet aggregation induced by Streptococcus suis serotype 2 muramidase-released pro-tein (MRP) / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 211-217, 2018.
Article
en Zh
| WPRIM
| ID: wpr-711391
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the mechanism of platelet aggregation induced by Streptococcus suis serotype 2 muramidase-released protein (MRP) and to provide scientific proof and theoretical basis for clinical treatment of patients with Streptococcus suis infection. Methods Nickel column affinity chromatogra-phy was used to purify recombinant proteins of MRP-N and MRP-C. Platelet aggregometer, thromboelastog-raphy (TEG) and scanning electron microscope were used to observe the platelet aggregation induced by MRP. Results Streptococcus suis 2 wild type strain,but not the mutant strain ΔMRP,could induce platelet aggregation. It was MRP-N but not MRP-C that induced platelet aggregation. GPRP,an inhibitor of β2inte-grin receptor,could significantly inhibit the platelet aggregation induced by MRP. Conclusion Streptococ-cus suis 2 MRP induces platelet aggregation through β2integrin receptor pathway.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Microbiology and Immunology
Año:
2018
Tipo del documento:
Article