Study on HPLC Fingerprint of Qingbi Granules / 中国药房
China Pharmacy
; (12): 4282-4285, 2017.
Article
en Zh
| WPRIM
| ID: wpr-704428
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE:To establish HPLC fingerprint of Qingbi granules.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consisted of methanol-0.3％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 260 nm,and column temperature was 25 ℃.The sample size was 10 μL.Using baicalin as reference,HPLC chromatograms of 10 batches of samples were determined.Common peak identification and similarity evaluation were performed by using TCM Chromatographic Fingerprint Similarity Evaluation Software (2.0 edition).RESULTS:There were 29 common peaks in HPLC chromatograms of 10 batches of samples.The similarity among the 10 batches was more than 0.90.After validation,HPLC chromatograms of 10 batches of samples were in line with control fingerprints.CONCLUSIONS:Established fingerprints can provide reference for identification and quality evaluation of Qingbi granules.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Año:
2017
Tipo del documento:
Article