Construction of Brucella Unmarked Deletion Mutant by Using Conventional Cloning Vector as Suicide Plasmid / 微生物学通报
Microbiology
; (12)1992.
Article
en Zh
| WPRIM
| ID: wpr-685555
Biblioteca responsable:
WPRO
ABSTRACT
Construction of mutant strain is an essential method in pathogenesis researches. The conventional method for Brucella unmarked deletion mutant construction is based on suicide plasmid, but the efficiency is very low. In the present study, we first optimized the electroporation parameters, and then, the cloning plasmid pEX18Gm containing sacB was successfully used to construct unmarked deletion mutant of the type IV secretion system. This indicated that by using conventional cloning plasmid as suicide plasmid in Brucella, unmarked deletion mutants can be constructed with high efficiency.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Microbiology
Año:
1992
Tipo del documento:
Article