EXPRESSION OF L-N-CARBAMOYLASE GENE IN PICHIA PASTORIS / 微生物学通报
Microbiology
; (12)1992.
Article
en Zh
| WPRIM
| ID: wpr-684360
Biblioteca responsable:
WPRO
ABSTRACT
N-carbamoylase is a part of hydantoinase operon which can transform N-carbamoylamino acid to corresponding ammo acids. The L-N-carbamoylase of Arthrobacter BT801, codied by the HyuC gene, is the rate-limiting and the only stereoselective enzyme. HyuC DNA fragment was amplified by PCR from the plasmid of pUC18-169. The target fragment was introduced into pPIC3. 5K plasmid to construct the pPIC3. 5K-hyuC expressing vector which was then transduced into Pichia pastoris GS115 cells after being linearized by BglⅡ digestion. Multi-copies insertion transformants were screened on G418 plates. The recombinant protein was proved to have biological activity of hydrolyzing N-carbamoylphenylalanine into phenylalanine through enzyme activity determination.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Microbiology
Año:
1992
Tipo del documento:
Article