Identification of the antigenic determinants of human augmenter of liver regeneration (hALR) recognized by its monoclonal antibodies / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2000.
Article
en Zh
| WPRIM
| ID: wpr-674899
Biblioteca responsable:
WPRO
ABSTRACT
Objective:Identification of the antigenic determinants of hALR.Methods:Theoretic antigenic determinants of hALR was predicted by using Hopp&Wodds method and and Goldkey software package.The four polypeptides according to the amino acid sequences of the predictive linear epitopes of hALR were synthesized and were used to analyze the antigenic determinants of hALR recognized by antibodies.Results:The polypeptides corresponding to residues 6～5,68～80 and 105～112 of hALR were its antigenic determinants.Conclusion:Prediction of the protein antigenic determinants by computer program and detection of them by competitive ELISA with synthesized polypeptides is a useful method of identification of the antigenic determinants.
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1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Año:
2000
Tipo del documento:
Article