Antisense oligonucleotide mediated inhibition on telomerase activity in gallbladder carcinoma cell / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
; (4): 27-30, 2005.
Article
en Zh
| WPRIM
| ID: wpr-671480
Biblioteca responsable:
WPRO
ABSTRACT
Objective:To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC-SD cell.Methods:We design the antisense,sense,and random oligonucleotide with phosphoric acid modification for the hTR(Human Telomerase RNA)template sequence.MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC-SD cell ,and fibroblast cells were used as control group.Results:PS-ODN can lead to the reduction of cell survival rate of GBC-SD cell,wich dosage dependence.Tne experimental group cell detected by scanning electron appeared apoptotic feature.Conclusion:PS-ODN can inhibit telomerase activity of GBC-SD cell effectively and induce the cell apoptosis.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Current Advances in General Surgery
Año:
2005
Tipo del documento:
Article