Inhibitory effect of curcumin on human breast cancer cell line SKBR3 and its mechanism / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
; (6): 15-17, 2017.
Article
en Zh
| WPRIM
| ID: wpr-657695
Biblioteca responsable:
WPRO
ABSTRACT
Objective To observe the effect of curcumin on proliferation, apoptosis, Caspase-3 activity and telomerase activity of chronic granulocytic breast cancer SKBR3 cell line, and to investigate the mechanism of curcumin. Methods The cell growth curve was drawn by MTT;the apoptotic cells were detected by Annexin, V and PI double staining; the expression of Caspase-3 was detected by Westernblot; the activity of telomerase was detected by TRAP-PCR silver staining. Results Curcumin could significantly inhibit the proliferation of SKBR3 cells in a time and concentration dependent, has a certain effect on reducing the telomerase activity of SKBR3 cells can enhance the expression of Caspase-3 induced apoptosis. Conclusion Curcumin can effectively inhibit the proliferation of SKBR3 cells, increase the synthesis of Caspase-3 protein, inhibit the activity of telomerase and mediate the apoptosis of SKBR3 cells.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Biochemical Pharmaceutics
Año:
2017
Tipo del documento:
Article