Construction of a Saccharopolyspora erythraea mutant producing erythromycin B as its major fermentation product / 军事医学科学院院刊
Bulletin of The Academy of Military Medical Sciences
; (6): 18-23, 2009.
Article
en Zh
| WPRIM
| ID: wpr-642686
Biblioteca responsable:
WPRO
ABSTRACT
Objective:To obtain a large amount of erythromycin B and to investigate the activity site in eryK. Methods:The key sequence of the BC loop region in eryK gene was knocked out and the eryK gene with 101 bp deleted was amplified by overlapping PCR,and cloned into vector pWHM3 to construct recombinant plasmid. The Saccharopolyspora erythraea mutant AK17 was constructed through chromosomal homologous recombination technique.Results and Conclusions:The S.erythraea mutant AK17 was constructed. The results of TCL and MS analysis showed that the major fermentation product of AK17 is erythromycin B.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Bulletin of The Academy of Military Medical Sciences
Año:
2009
Tipo del documento:
Article