Identification and analysis of arsenic interactors byEscherichia coli proteome microarray / 上海交通大学学报(医学版)
Journal of Shanghai Jiaotong University(Medical Science)
; (12): 583-587, 2017.
Article
en Zh
| WPRIM
| ID: wpr-610484
Biblioteca responsable:
WPRO
ABSTRACT
Objective · To globally study the influence of arsenite to the various biological pathways of Escherichia coli as a model organism.Methods · The protein-arsenite interactions was globally studied based on a proteome microarray constructed by 4256 affinity-purified Escherichia coli proteins. The functions of interacting proteins and their network were then analyzed by bioinformatics. Results · 91 proteins that remarkably interact with arsenic were successfully identified. Bioinformatics analysis found that most of the proteins possess catalytic activityand are involved in various biosynthesis and cellular metabolism pathways. The interactions of arsenic with proteins encoded by malY, cfa and hypF genes were further validated by Western blotting, which proves the results of proteome microarray reliable. Conclusion · Arsenite interacts with a variety of enzymes ofEscherichia coli and can greatly affect its biological metabolism.
Texto completo:
1
Base de datos:
WPRIM
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Journal of Shanghai Jiaotong University(Medical Science)
Año:
2017
Tipo del documento:
Article