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Application of Autoradiography in Study of Cold Storaged Transplant Organs / 中国医科大学学报
Journal of China Medical University ; (12): 101-102,107, 2001.
Article en Zh | WPRIM | ID: wpr-585332
Biblioteca responsable: WPRO
ABSTRACT
Objective: Our purpose was to study whether exogenous adenosine triphosphate (ATP) could enter liver cell or not. Methods: Using continuous hypothermic machine perfusion model of rat liver, we used autoradiography in this experiment. Results: The light microscope pictures of group A(no [α-32P] ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq [α- 32P]ATP in the perfusate) showed that distribution of numerous [α-32P]ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel. Conclusion: Exogenous ATP could enter cold storaged rat liver cell.
Texto completo: 1 Base de datos: WPRIM Idioma: Zh Revista: Journal of China Medical University Año: 2001 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Idioma: Zh Revista: Journal of China Medical University Año: 2001 Tipo del documento: Article