The fitting and optimization of standard curve for determining concentration of serum hepatitis B virus large surface protein with program solution of Excel / 中国实用内科杂志
Chinese Journal of Practical Internal Medicine
; (12)2006.
Article
en Zh
| WPRIM
| ID: wpr-563766
Biblioteca responsable:
WPRO
ABSTRACT
Objective To discuss a convenient and pragmatic method of fitting and optimizing standard curve for determining concentration of serum hepatitis B virus large surface protein(HBV-LP).MethodsEnzyme-linked immunosorbent assay(ELISA)was used to measure the absorbance of standard preparation of HBV-LP.Concentration and absorbance of standard preparation of HBV-LP was carried out curve fitting with 4-parameter formula model and linear model and log-linear model and quadratic polynomial model and cubic polynomial model and S model by program solution of Excel,respectively.The most standard curve for determining concentration of serum HBV-LP was determined with coefficient of determination of regression model.ResultsThe scatterplot of standard preparation of HBV-LP submited nonlinear tendency.There were all significance to regression equation of 4-parameter formula model and linear model and log-linear model and quadratic polynomial model and cubic polynomial model and S model(P
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Practical Internal Medicine
Año:
2006
Tipo del documento:
Article